P11 is a 3rd generation synchrotron beamline for High-throughput Macromolecular Crystallography (MX). It is energy tunable in the range 5.5-26 keV and has adaptable optics for focused beam size varying from 200 µm to 5 µm, with a maximum flux of 1013 photons/second. The large capacity sample changer can hold 368 samples in Unipuck format. The state-of-the-art photon counting detector, Eiger2 X 16M, also contributes to the high throughput of the beamline, at which 720 samples can be measured per day, making it ideal for large scale campaigns. Experimental phasing is supported with fluorescence detector. The capabilities of the beamline allow serial crystallography which is realised using a tapedrive as sample delivery and facilitated by auto-processing routines. In classical MX, the beamline can be operated remotely, and unattended data collections are under development. The beamline is supported by a user laboratory that includes OLTShifter for semi-automated crystal harvesting.
DESY offers project-based access requiring more scientific support than the usual general access shifts at the beamline, during which the guest scientists prepare and carry out themselves and are supported by beamline staff mainly in the use of the beamline instruments only. Expanded projects could vary from assistance in classical MX to sophisticated or laborious experiments in serial crystallography, phasing and fragment screening, all the way to the preparation of large-scale experiments in life science techniques, using multiple disciplines. Our staff can assist with sample preparation, data collection, data analysis and project preparation to
include and merge other techniques.
DESY P11 provides access to the macromolecular crystallography beamline as remote or onsite experiments for all available experiment types. Each shift contains scientific and technical assistance.
P11 https://photon-science.desy.de/facilities/petra_iii/beamlines/p11_bio_imaging_and_diffraction/index_eng.html
Physical access: yes
Remote access: yes
Average time of a visit:1 shift, 8 h
Information about sample preparation (if relevant): preprepared samples shipped in unipucks.
High-throughput Macromolecular Crystallography beamline P11, PETRA III, DESY
Notkestraße 85
22607, Hamburg
Germany
