Purification strategies

Here is a linkage to a resource of information in the Website of The Protein Purification Facility (Dr. Mario Lebendiker) Hebrew University of Jerusalem.

(Sub-topics in the field are under construction)

Go back to the P4EU Resource page

1. Purification Strategy: Handbooks, Technique selection guides, Columns, Buffers, Virus Purification, etc. PROTOCOLS
2. Preparation of Cell Lysates / Different Disruption Methods. Protease Inhibitors. DNA removal. Endotoxin removal. Detergents. PROTOCOLS
3. Purification of Recombinant Proteins: poly His-Tagged, GST, MBP, Strep-tag, Calmodulin, Intein, CBD, SUMO, etc. PROTOCOLS
4. Cleavage of Recombinant Proteins: Thrombin, Factor Xa, TEV-Protease, Enterokinase, SUMO, PreScission, TAGZyme, etc. Proteases sensitivity to Detergents. PROTOCOLS
5. Conventional Chromatography: Gel Filtration, Ion Exchange, Hydrophobic Interaction, Multimodal, Hydroxyapatite, Reverse Phase Chromatography, Chromatofusing, Thiophilic.
6. Affinity Chromatography: Activated Resins, Antibody Purification and antibody fragmentation, Lectins and Glycoprotein Purification, Deglycosylation, Immunoprecipitation. PROTOCOLS.
7. Other Purification Procedures: Desalting, Ultrafiltration, Concentration, Dialysis, Protein Quantitation. PROTOCOLS.
8. Protein Aggregation: Kits. PROTOCOLS
9. Gel Electrophoresis: PAGE-SDS, Native gels, Gel Stain, Western, etc. PROTOCOLS