Purification of Recombinant Proteins

Linkage to a resource of information about Poly His-Tagged Fusion Proteins from different companies: System user Manuals, resin properties, etc

PROTOCOLS

1. Small and large scale His-Tag fusion protein purification under nature conditions.The Protein Purification Facility of The Hebrew University of Jerusalem
2. Bacterial Expression Screen - DDM (Dodecyl Maltoside) lysis - Small Affinity binding. The Protein Purification Facility of The Hebrew University of Jerusalem
3. Batch Protein Refolding on IMAC resin. The Protein Purification Facility of The Hebrew University of Jerusalem

Glutathione-S-transferase (GST) tagged fusion proteins

Linkage to a resource of information about GST -Tagged Fusion Proteins from different companies: System user Manuals, resin properties, etc

PROTOCOLS

1. Small Scale GST-Tag Purification Under Nature Conditions. The Protein Purification Facility of The Hebrew University of Jerusalem

Maltose Binding Protein (MBP) tagged fusion proteins

Linkage to a resource of information about MBP -Tagged Fusion Proteins from different companies: System user Manuals, resin properties, etc

PROTOCOLS

1. Small and Large Scale MBP-fusion Protein Purification. The Protein Purification Facility of The Hebrew University of Jerusalem

SUMO tagged fusion proteins

Linkage to a resource of information about SUMO Fusion Proteins from different companies: System user Manuals, resin properties, etc

PROTOCOLS

Strep-tag fusion proteins

Linkage to a resource of information about Strep-tag Fusion Proteins from different companies: System user Manuals, resin properties, etc

PROTOCOLS

Other fusion proteins

System user Manuals, resin properties, different companies, etc

Calmodulin Binding Peptide

Intein Mediated Purification

T7-tag

Cellulose Binding Domain (CBD)

NusA fusion protein

Biotinylated fusion protein

FLAG-tag

HaloTag

Cold Expression

Profinity eXact fusion-tag system

PROTOCOLS

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