Hi everyone,
I have a colleague who is looking for a pTriEx based vector with an N-terminal His tag followed by a 3C precission protease site. Also it would be great if it is LIC compatible. Does anyone have such a vector that they could provide?
thanks!
best,
peggy
Hi Peggy,
pOPINF (Oxford or Addgene) fits the bill. It is based on pTriEx2 and has an MAH6ssglevlfq/gp tag. It can be used for In-Fusion or SLIC but not classical LIC with the base 'lock' due to the base composition of the extensions.
Nick
Hi Nick,
Thanks - I'll forward that information!
peggy