We are pleased to invite you to the next DRIIMB Webinar, featuring Prof. Stephen Muench from the School of Biomedical Sciences, University of Leeds.
Title: Development, uses and future directions for time-resolved single particle CryoEM
Date: Wednesday, 29 July
Time: 14:00–15:00 BST (UK)
Registration link
Abstract
The Muench group have been developing new approaches for both sample preparation and time-resolved studies within single particle cryoEM. The time-resolved methodologies can mix and freeze a sample from the micro-second timescale, but challenges still remain in obtaining good ice quality and studying longer timescales >200 ms without the thin film environment influencing the results. Moreover, many devices require a complex setup, and uptake by the wider community has been slow.
In this webinar, Prof. Muench will discuss some of their new approaches including a simplified approach to make experiments easier and present recent work using time-resolved cryoEM. With advances in complementary techniques such as time-resolved X-ray crystallography, NMR spectroscopy, and hydrogen–deuterium exchange (HDX) mass spectrometry, we have a rich toolkit that can investigate a broad range in both time and length scales. The talk will also explore how time-resolved EM might fit within this broader structural biology landscape.
Speaker
Prof. Stephen Muench is Professor of Membrane Biology and Structural Biology at the University of Leeds. His group has worked on a range of different systems and technologies from developing new small molecules, membrane protein scaffolds, biosensors and time-resolved approaches. Major contributions to the field include: (i) The use of EM to drive inhibitor design for membrane proteins such as TRPC1 and the bc1 complex, (ii) development of time-resolved methodologies for cryoEM, (iii) better understanding of sample preparation within single particle cryoEM, (iv) work on SMA polymers and hybrid vesicles with block co-polymers to improve membrane protein lifetimes. More details can be found here.
Click here to register for the webinar!
We hope you will be able to join us for what promises to be an engaging and informative seminar.
