The 3rd one-week practical course on Image Processing for Electron Microscopy focused on (Cryo-)Tomography (IPEMtomo) will be held at Institut Pasteur, Paris, November 6-10, 2023.
Electron tomography combined with image processing is one of the most powerful methods for studying cellular details in 3D. In situ studies can reveal additional important information about macromolecules in their complex environment and adopted conformations.
This course aims to introduce basic concepts in image analysis and practical experience in tomographic reconstructions for different needs: processing of low-dose tilt series with and without fiducials for 3D volume annotation or subtomogram averaging. An introduction to subtomogram averaging and initial model generation steps will be covered.
The course is aimed at PhD students, post-doctoral fellows, engineers, and researchers who have some experience in (Cryo-)EM and would like to use (Cryo-)ET in the near future. A maximum of 12 participants will be selected. Applicants who already have tilt-series for processing will be prioritised.
