CEITEC is a node of the Czech Infrastructure for Integrative Structural Biology, which provides services for molecular biology, biochemical and structural characterization of cellular processes research.
The Czech Infrastructure for Integrative Structural Biology (CIISB) is formed by two Centers of Excellence for Structural Biology constructed within the projects CEITEC – Central European Institute of Technology, Brno and BIOCEV - Biotechnology and Biomedicine Centre, Vestec, Prague-West. The Czech structural biology community is represented by the Czech Society for Structural Biology (CSSB), which is forming a national link to Instruct ERIC. CIISB affiliation with Instruct ERIC contributes to the development of human resources in research, attracts qualified national and international researchers, and enables efficient dissemination of knowledge and expertise, as well as efficient use of the infrastructure.
CEITEC is a young and dynamic centre of scientific excellence which strives to fulfill its mission in advancing knowledge in the field of life sciences, advanced materials, and nanotechnologies. Six Integrative Structural Biology Core Facilities of total 12 core facilities are equipped with cutting-edge technologies and are accessible to internal and external users from academy and industry nationally and internationally.
Cryo-electron microscopy and tomography core facility provides access to state-of-the-art electron microscopy instrumentation, including Titan Krios, Talos Arctica, and more, for structural and cellular biology research. The other facilities also provide services for the studies of structure, dynamics and interactions of biomolecules, as well as for biophysical and structural characterisation of biomolecules, and studying (bio)molecular interactions, etc. The measurements can be performed in person as well as fully remotely. All facilities support users in project planning, sample preparation, and data analysis.
CEITEC is currently available for physical as well as remote access. For more information on the services available, please check the Service/Technology pages.
CEITEC is located on Masaryk University Campus in the southwest part of Brno, Czech Republic. The site can be easily reached by car, bus or train. More detailed information about how to get to CEITEC is available here. Visitors can be accommodated at the University hotel, apartments on nearby Studentska street or various city hotels.
Recent covid information is available here.
The Cryo-electron microscopy and tomography core facility (CEMCOF) at CEITEC Instruct centre is an open access facility providing access to state-of-the-art instrumentation for cryo-electron microscopy, support with cryo-EM sample preparation, and data analysis. The facility handles BSL1 and BSL2 samples, respectively, and is available to its users for both service and collaborative projects.
More information can be found on the Cryo-electron microscopy and tomography core facility (CEMCOF) webpage
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The platform at the Biomolecular Interactions and Crystallography core facility (BIC) at CEITEC Instruct centre provides services for biophysical and structural characterisation of biomolecules, and studying (bio)molecular interactions. It is equipped with the instrumentation to set up crystallisation conditions of biomolecules and their complexes, basic characterisation of physical properties of the molecules (analytical ultracentrifugation, dynamic light scattering, CD spectroscopy, differential scanning calorimetry, differential scanning fluorimetry, size-exclusion chromatography), and to study thermodynamics and/or kinetics of interactions (isothermal titration calorimetry, surface plasmon resonance, bio-layer interferometry, microscale thermophoresis, CD spectroscopy, analytical ultracentrifugation). The facility is accessible each working day upon previous agreement.
More information can be found on the Biomolecular Interactions and Crystallography core facility webpage.
The platform at the Biomolecular Interactions and Crystallography core facility (BIC) at CEITEC Instruct centre provides instruments and services leading to the biophysical characterization of (bio)molecules, the study of biomolecular interactions (determination of affinity, kinetic and thermodynamic parameters) and the sample quality control using analytical ultracentrifugation (AUC), isothermal titration calorimetry (ITC), surface plasmon resonance (SPR), bio-layer interferometry (BLI), microscale thermophoresis (MST), dynamic light scattering (DLS), differential scanning calorimetry (DSC), differential scanning fluorimetry (DSF), and analytical size-exclusion chromatography (SEC-MALS). The platform is available to the entire INSTRUCT-ERIC community every working day upon previous agreement.
More information can be found on the Biomolecular Interactions and Crystallography core facility webpage
The Nanobiotechnology Laboratory is a shared laboratory at the CEITEC Instruct centre helping other scientists, such as biochemists, chemists and biologists, to better understand the biological processes taking place in living cells, but also to image biomolecules in their natural environment, or complex properties of new nanomaterials. Our labs contribute to a better understanding of heart diseases, the effects of cytostatics on cancer cells or to the development of new treatments of osteoarthritis. We monitor the structure of biomolecules and nanomaterials for the preparation of new types of biosensors. Monitoring cell adhesion then contributes to the development of new implant materials. The main instruments used in our laboratories are AFM microscopes, confocal optical microscope, Raman microscope, biosensor platforms, electrochemical and electrophysiological instruments.
More information can be found on the Nanobiotechnology core facility (NANOBIO) webpage.
The Proteomics core facility at CEITEC Instruct centre provides the academic and other entities with access to advanced proteomic technologies based on shared resources and the know-how of highly trained staff. Effective utilization of the state-of-the-art mass spectrometric instrumentation promotes understanding proteome complexity according to the demands of research community. Thus, the facility is involved in broad range of projects requiring protein characterisation covering fields of biochemistry, molecular and structural biology, human and veterinary medicine, microbiology, plant, agriculture and food sciences. The facility staff can offer a complete mass spectrometry-assisted proteomics services including sample preparation, separation of protein/peptide mixtures, qualitative and quantitative characterisation of proteins and their modifications by mass spectrometry and data processing.
More information can be found on the Proteomics core facility webpage.
The Core Facility of High Field NMR Spectroscopy at CEITEC Instruct centre provides access to NMR spectrometers in the range of proton frequencies from 500 MHz to 950 MHz. The equipment is suited mainly to the studies of structure, dynamics and interactions of biomolecules, i.e. proteins, nucleic acids and carbohydrates. However, the instrumentation is flexible enough to cover various research needs in material science, organic and inorganic chemistry, biochemistry, biology and biophysics.
More information can be found on the Core Facility of High Field NMR Spectroscopy webpage
X-ray Diffraction and Bio-SAXS laboratories are a part of Biomolecular Interactions and Crystallography core facility (BIC) at CEITEC Instruct centre. The laboratories are equipped with top-class instruments for diffraction experiments with single crystal samples focused on the determination of the 3-D structure of (macro)molecules down to atomic resolution and for small angle X-ray scattering experiments with isotropically scattering samples focused on determination of the shape and size of macromolecules or nanoparticles.
Services include diffraction/SAXS data collection, analysis and structure determination.
More information can be found on the Biomolecular Interactions and Crystallography core facility webpage.
