11-Dec-2024
The Instruct Centre FR2 in Grenoble has recently added to its fleet a powerful Titan Krios G4 cryo-electron microscope (Thermo Fisher Scientific), equipped with a cold FEG, a Selectris X energy filter and a Falcon 4i electron detector. Part of the EquipEx+ France Cryo-EM project, financed by the Plan Investissement d’Avenir 3, that has enabled the purchase of three state-of-the-art 300 kV cryo-TEMs in France, the Grenoble installation, designated CM02, will serve the French community and its European partners.
CM02 is an ESRF CRG beamline operated by a team of 5 people from the IBS-ISBG (Grenoble) with Guy Schoehn as the scientific director. CM02 is accessible to academia and industry for on-site visits or remote experiments. Evidence of sample quality and suitability must be provided to justify access to this microscope. All are strongly encouraged to get in touch and discuss with the team prior to their first application.
Instruct-ERIC can provide funded access to CM02 (including sample shipment and travel expenses) for laboratories located at any Instruct-ERIC member state or organisation (and can provide self-funded access for researchers who are not in current member countries). Note that the Instruct-FR2 platforms, as well as those of other Instruct Centres, can provide expertise and a full suite of tools, enabling seamless workflows from sample pre-characterisation to sample optimisation, prior to high-end data acquisition on CM02, for single-particle and tomography experiments. Please contact the IBS-ISBG EM platform to discuss in detail or apply via ARIA.
Other routes for access include National CRG access, reserved for laboratories located in France (please contact the CM02 team directly), ESRF access where applications are made through the ESRF User Portal (SMIS), and also Industrial access (please contact the team for a quote).
The CM02 TFS Titan Krios G4 installation in Grenoble and Apoferritin particles embedded in vitreous ice, eventually generating a 3D reconstruction at a resolution below 1.3 (on site manufacturer's test).
The CM02 team would like to express its gratitude and a warm thank you to Guy Schoehn (IBS) for the establishment of the EM facility and to everyone involved in the Grenoble branch of the « France Cryo-EM » project, at the management level at the IBS, ISBG and ESRF, at the construction level (ESRF) and to the TFS engineers who reassembled the instrument.
Find out more about Instruct-FR2 and apply to access their technologies here.
