Dear All,

after having purified a secreted protein in insect cells, where its native SS was not correctly processed, we plan to change to that design: gp67 SS - StrepTag - Sumo Star Site - target protein.

Has anyone positive or negative experience with that setting?

Best

Sabine

Hi Sabine

You probably know about this, but just in case, there is an on-line signal sequence predictor that could be of use here.

http://www.cbs.dtu.dk/services/SignalP/

Best

Bjørn

Hi Sabine

You probably know about this, but just in case, there is an on-line signal sequence predictor that could be of use here.

http://www.cbs.dtu.dk/services/SignalP/

Best

Bjørn