Dear All,

has anyone experience in preventing protein glykosylation in baculovirus expressed protein for crysallization (instead of enzymatic deglyosylation of the purified protein) ?

Best

Sabine

Hi Sabine:

we have very good results in a project with a glycosilated protein from insect cells, and I perform a very high resolutive Cation exchange  to separate the different glycosilated forms. In our case our protein precipitate after deglycosilation, so separate the different isoforms is the only way. 

Mario

Hi Sabine,We use tunicamycin in our baculovirus production during the expression of the protein.Christine