Overview:
This project to be developed at the São Carlos Institute of Physics (IFSC), University of São Paulo (USP) in Brazil, aims to study the in situ structure of mitochondrial filamentous enzymes involved in the metabolic adaptation of tumors and their effects on organelle ultrastructure and morphology upon filamentation. This research will be an extension of Adamoski et al., NSMB 2023 - which was recently highlighted in the celebration of NSMB´s 30th anniversary - now focusing on more profound questions with cutting-edge methodologies, such as correlative light-electron microscopy (CLEM), cryo-electron tomography in lamella (cryo-ET) and expansion microscopy (ExM), that can also be applied to other proteins in the lab.
Methodological Responsibilities:
1) Sample Preparation: Engage in cell culturing, vitrification, and lamellae preparation in our lab at the IFSC/USP;
2) Data Collection and Processing: Perform high-resolution tomographic data collection using top-tier microscopy facilities in Brazil and internationally. Data processing will be done in-house.
Requirements:
1) PhD in Chemistry, Biochemistry, Molecular Biology, or related;
2) Experience in Cell Biology; proficiency in cryo-FIB and cryo-ET, or single-particle cryo-EM is a big plus;
3) Strong analytical, collaboration, and English language skills.
This opportunity is open to candidates of any nationality. The selected candidate will receive a Postdoctoral fellowship from the São Paulo Research Foundation (FAPESP) for R$ 12,000 monthly and a research contingency fund, equivalent to 10% of the annual value of the fellowship, allocated for research-related expenses.
