Hi everybody,

welcome to our first forum discussion on the P4EU networking site. Let me know if you can read this and what you think about it so far.

Hüseyin

Hi Hüseyin,

I can read your comment. At least in my browser window, the text I type in is microscopic - I'll see if that changes when I submit.

Would be also good to get either email or rss alert to new discussions in a thread. This is common on forums, but doesn't seem to be implemented here.

Darren

Well the text looks OK once submitted, but is hard to read as I type. Will try a different browser (FF7 on Linux here).

Hi Darren,

thanks for testing the forum. I totally agree that we should get an update message during a discussion. Makes no sense otherwise if you wait for someone to remember the discussion.

I will ask Jane if that can be added here as well.

Hüseyin

Darren and his browsers.... ;) Actually Darren - it is the same issue here when I type in the message area. I'll put a bug in to the Developers.

All the best, Jane

Thank you to the developer who fixed it.

Jane

I can see all the comments well. This is the first view to the page. I agree with Darren. I never used RSS feeds or alerts before. How do you get this message and where?

Who is able to see this first discussion.

For the record. Is anyone having a decent stock of Rosetta 2 (DE3)? We get weird results with the Novagen material. Cells do not grow in larger volumes (300 ml)?

Sincerely yours

Joop

Hi all,

I can see the whole thread with good font size... All this looks promising.

Regards

Renaud

Hi Joop,

other the years we had 2-3 issues with our competant cells, on each time we bought a new stock from Novagen and made new stocks starting from these, this always solved the problem. I would advise you to spend the few hundreds euro to be on the safe side. I don't use the Ros (DE3) but only the Ros (DE3)pLys S, if you want I can send you an aliquot.

Renaud

It would be nice if you could send me a stab culture or  a plate. We actually bought a new batch of Ros2 (DE3) competent cells.

We don't know what is wrong yet.

Best and Thanks Joop