Instruct PartnerReNaFoBis together with FRISBI will organize a webinar:
November 14, 2024 at 01pm: 2 speakers
Lionel Imbert, IBS, Grenoble, France Title: Overview of cell-free protein production & developments for NMR samples
Summary: Cell-free expression is used to produce protein samples for structural biology studies. It uses the translation and transcription machinery of E. coli to synthesize proteins in an open environment, i.e. without the bacterial cell wall. For NMR applications, isotopically-labelled amino acids can be used directly in the reaction to synthesize only the protein of interest. Rapid production and simplified purification processes have enabled many structures to be solved by NMR, but there are a number of limitations. Firstly, the size of the proteins to be studied with uniform labelling cannot exceed 20 kDa. Secondly, for higher molecular weight proteins, a larger quantity of protein needs to be synthesized at reasonable cost. Developments made on the IBS/ISBG cell-free platform to perform perdeuteration in water, selective protonation, therapeutic antibody fragment production and position-specific labelling will be among others presented.
Martin Pelosse, EMBL Grenoble Title: Eukaryotic expression strategies at the EEF.
Summary: Structural and biochemical characterization of large protein complexes is a prerequisite for better understanding biological functions at a molecular level. However recombinant production of such large samples can sometimes be cumbersome. At the EEF, users have access to modular cloning systems facilitating the assembly of large expression vectors as well as different strategies for the expression of their protein complexes. Additional tools completing traditional baculovirus expression vector systems (BEVS) and developed at the EEF, will be introduced.
Registration: https://us06web.zoom.us/webinar/register/WN_jtzZvMCbRT2cwq3lZWX_PQ
Organiser: Arnaud Poterszman
