NCCAT will be offering a 1 week workshop focused on the theory and practice of single-particle analysis. The mornings will have lectures and stimulating round table discussions. The afternoons provide hands-on practicals to reinforce fundamental concepts and topics covered earlier in the day.
Day 1: Monday, March 2, 2020
Morning
Roundtable: Welcome
Lecture 1: Keynote – Intro and overview of SPA – Joachim Frank (Columbia University)
Afternoon
Lecture 2: cryoVR sample preparation – Brenda Gonzalez & Jiahui Dong (Purdue University)
Practical 1: Support films and grids
Practical 2: Plunge freezers and Chameleon
Day 2: Tuesday, March 3, 2020
Morning
Lecture 3: Microscopes – SEMC staff
Roundtable: Facility building
Afternoon
Practical 3: TEM microscopes
Day 3: Wednesday, March 4, 2020
Morning
Lecture 4: Algorithms and foundational math – Fred Sigworth (Yale University)
Lecture 5: Data Analysis and reconstruction workflow – Amedee des Georges (ASRC/CUNY)
Roundtable: Current algorithms to deal with SPA
Afternoon
Practical 4: SPA RELION/cryoSPARC (in the cloud using AWS)
Day 4: Thursday, March 5, 2020
Morning
Lecture 6: Interpretation and Limitations – Rich Hite (Memorial Sloan Kettering Cancer Center)
Lecture 7: Validation Methods – Tom Walz (Rockefeller University)
Roundtable: EM challenges and new frontiers
Afternoon
Practical 5: SPA RELION/cryoSPARC (in the cloud cont)
Lecture 8: EMDataResource: Structure Data Archiving, Validation Challenges – Cathy Lawson (Rutgers University)
Practical 6: Validation methods – 3DFSC
Evening
Group dinner
Day 5: Friday, March 6, 2020
Morning
Lecture 9: Moderate resolution interpretation – Gira Bhabha & Damien Ekiert (New York University)
Lecture 10: Fitting Atomic Models – Oli Clarke (Columbia Univerisity)
Roundtable: Making biological conclusions from cryoEM reconstructions.
Afternoon
Practical 7: Coot and model building
Roundtable: Farewell
For further information or to apply for a place, click here.
