This two-day training event will discuss strategies to engineer sufficiently pure, homogenous and high-quality recombinant proteins that are critical for subsequent structural and functional studies. The event, aimed at early career researchers will cover the latest bioinformatic tools for construct design and protein characterisation, tips and tricks for improving protein expression, purification, stability/preparation and evaluating quality with biophysical techniques.
The event will be interactive with opportunities to bring proteins to analyse on some of the latest instrumentation/software and to present a poster or talk on these topics so you can troubleshoot your protein challenges with us.
Topics will include:
• Protein Bioinformatics
• Protein Expression
• Protein Purification
• Biophysical Characterisation
Key learning objectives:
1. To be able to demonstrate knowledge of the full pathway of protein construct design, expression, purification and characterisation.
2. To be able to describe the principles and applications of bioinformatic and biophysical approaches to their research and have a chance to collect/analyse data on their protein of interest.
3. To understand how to optimise and evaluate protein expression and purification.
Programme Coordinators for this event are:
Nicola Burgess-Brown, Protein Sciences, Structural Genomics Consortium
Rivka Isaacson, Kings College London
Elliott Stollar, University of Liverpool
Limited spaces, register now!
