As the UK EM Validation Network, we have organised this meeting to present current thinking on cryo-EM map and map/model validation, and to consider future research directions that anticipate the growing needs in this area. The community effort to apply cryo-EM to structural proteins of SARS-CoV-2 has been a success story but has also highlighted the importance of structure validation.
Each day will consist of two sessions with talks and a panel discussion, with the following themes:
Day 1: 18th Nov Analysis and validation of SARS-CoV-2 EM structures
Day 2: 19th Nov Single particle map and tomogram validation
Day 3: 20th Nov Map-model validation
The meeting organisers are supported by a technology-development grant from the Wellcome Trust. An earlier (in-person) workshop held in January 2019 at EMBL-EBI was a great success and we expect the November meeting to be equally interesting and to reach a considerably larger audience through the online format.
Scientific organisers
Gerard Kleywegt (EMBL-EBI)
Garib Murshudov (MRC-LMB)
Elena Orlova (Birkbeck College)
Ardan Patwardhan (EMBL-EBI)
Alan Roseman (University of Manchester)
Peter B. Rosenthal (The Francis Crick Institute)
Maya Topf (Birkbeck College)
Martyn Winn (STFC)
Registration website
Please register in advance of the meeting here. Registration is free of charge, but is required for attendance.
How it will work
The main sessions and panel discussions will take place via Zoom. A link to join will be sent to you before the meeting.
We have also set up a Discord server for the meeting. Discord is a platform for text and video discussions which is very popular in the gaming community. For this meeting, the server will enable discussions to continue outside the Zoom sessions. We will support general discussions, as well as channels for ad hoc groups of people.
Programme
Note that all times are in UK local time (GMT). Talk titles and exact times will be added as they are confirmed. A full schedule will be available for download nearer to the meeting.
Wednesday 18th November - Analysis and validation of SARS-CoV-2 EM structures
14:00 - 17:15 Main session
Peter Rosenthal (Crick)
Ardan Patwardhan (EMBL-EBI) "SARS-CoV-2 structures in EMDB and EMPIAR"
Patrick Cramer (MPI Göttingen) "Structural studies of SARS-CoV-2 polymerase and mechanism of remdesivir inhibition"
Christiane Schaffitzel (Bristol) "A free fatty acid binding pocket in the locked structure of SARS-CoV-2 spike protein"
Beata Turoňová (EMBL) "In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges"
Donald Benton (Crick) "Structural basis of SARS-CoV-2 Receptor Binding"
Andrea Thorn (Hamburg) "Findings from the Coronavirus Structural Task Force"
Tristan Croll (Cambridge) "Rebuilding SARS-CoV-2 Cryo-EM structures with ISOLDE"
Agnel Joseph (STFC) "Reprocessing and validation of structures from SARS-CoV-2 and a web service for mapping genome mutations"
17:30 - 18:00 Panel discussion
Thursday 19th November - Single particle map and tomogram validation
14:00 - 17:15 Main session
Dmitry Lyumkis (Salk)
Tim Grant (UW-Madison)
Carsten Sachse (Jülich)"Significance and confidence of cryo-maps and resolution values"
Sjors Scheres (MRC-LMB Cambridge) "Single-particle cryo-EM to atomic resolution"
Daven Vasishtan (Oxford)
Niko Grigorieff (Brandeis) "Locating molecular assemblies by template matching in 2D and 3D: a comparison"
Giulia Zanetti (Birkbeck) "Common issues in subtomogram averaging: my perspective on current needs and validation"
Mauro Maiorca (Birkbeck)
17:30 - 18:00 Panel discussion
Friday 20th November - Map-model validation
14:00 - 17:15 Main session
Martyn Winn (STFC) "Cryo-EM validation tools in CCP-EM"
Keitaro Yamashita (MRC-LMB Cambridge) "Refinement of high resolution cryo-EM SPA structures with symmetry"
Rangana Warshamanage (MRC-LMB Cambridge) "EMDA validation tools as applied to recent SARS-CoV-2 Structures"
Andrei Istrate (EMBL-EBI) "Strudel - model-dependent map-feature validation and application to EM SARS-CoV-2 structures"
Paul Adams (LBL) "Cryo-EM tools in Phenix for validation and beyond"
Gerard Kleywegt (EMBL-EBI) "Validation at EMDB and wwPDB"
Cathy Lawson (Rutgers) "EMDataResource Validation Challenges: Overview and Outcomes"
Zhe Wang (EMBL-EBI) "EM Validation analysis from EMDB"
Valeriy Titarenko (Manchester) "Global and local model fitting and scoring for cryo-EM maps"
Wah Chiu (Stanford) "Quantitative Measure of Resolvability of CryoEM Density"
17:30 - 18:00 Panel discussion
Find out more on the CCPEM website.
