FC-ULISBOA

Portugal Research Site

FT-ICR and Structural MS Centre – FC-ULISBOA

The centre’s expertise is native MS and top-down analysis of proteins and protein complexes through Fourier transform ion-cyclotron resonance mass spectrometry. FT-ICR or magnetic resonance mass spectrometry, MRMS, offers extreme mass accuracy, unmatched resolving power and the broadest array of MSMS methods for protein structure interrogation. The centre can map complex PTM patterns in large proteins, characterise modified antibodies as well as macromolecular complexes composition, stoichiometry, stability, and connectivity, from minute amounts of sample. The centre offers internships to conduct research projects that require FT-ICR and hosts training events focused on mass spectrometry in the framework of integrative structural biology.

Flagship Technology at FC-ULISBOA:

The lab hosts three state of the art mass spectrometers for structural biology, a Magnetic Resonance Mass spectrometer, equipped with ParaCell and with the most up to date electronics and ion-optics, a modified Q-ToF for native MS studies, and a high performance, ETD capable Orbitrap, well suited for top-down protein analysis.

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