27-May-2022
The CERM/CIRMMP infrastructure, located in the Scientific Campus of the University of Florence, hosts the Instruct-ERIC Center-IT.
CERM/CIRMMP offers a wide range of structural biology services for Instruct users, including protein production in mammalian cells for in-cell NMR.
The video protocol in JoVE Journal recently published by CERM/CIRMMP researchers (Barbieri, L., & Luchinat, E. (2021). J. Vis. Exp, 169, e62323. DOI: 10.3791/62323) takes users through the process of setting up the NMR bioreactor for protein studies, providing a detailed overview of how the in-cell NMR service works.
This protocol describes the setup of an NMR bioreactor to keep encapsulated human cells viable for up to 72 h, followed by time-resolved in-cell NMR data acquisition and analysis. The methodology is applied to monitor intracellular protein-ligand interactions in real time.
Find out more about NMR services at Instruct-Italy, or any other services at CERM/CIRMMP to which Instruct members can acquire funded access.
